3D PSF Models for Fluorescence Microscopy: Implementation and Super-resolution Applications
Seminar • 28 November 2011 • BM 4.233AbstractI will introduce realistic 3D PSF (Point Spread Function) models for the wide-field and the confocal microscopes, and will show their potential use in super-resolution fluorescence microscopy. The presented work consists of model formulation by means of readily available microscopic parameters, and of a fast and accurate open source implementation in ImageJ. I will demonstrate the usefulness of the proposed models in point-source localization and in super-resolution data simulation. The proposed PSF-based approach is complementary to currently available algorithms that rely on simplified PSF models. The latter can be used for obtaining preliminary results and for having an immediate feedback about the quality of the experiment, while the more realistic models can be used for a more accurate analysis that will be performed at a later stage. Joint work with François Aguet and Daniel Sage.