Benchmarking of SMLM Software
Comprehensive review of the single-molecule localization microscopy (SMLM, PALM, STORM) software packages.
The second edition of the challenge will be held in the Single Molecule Localization Microscopy Symposium (SMLMS) at Lausanne, Switzerland, August 28-30, 2016. This edition will be focussed on both 3D localization techniques. Broad participation is warmly encouraged!Read more
D. Sage, H. Kirshner, T. Pengo, N. Stuurman, J. Min, S. Manley & M. Unser, Quantitative evaluation of software packages for single-molecule localization microscopy, Nature Methods 12, 717–724 (2015).Read full PDF
The Grand Challenge "Localization Microscopy" was presented as a workshop in the conference IEEE ISBI 2013 at San Francisco. Nearly 30 software have been run, mostly by the authors of the software, thus constituting the first world-wide effort in benchmarking the SMLM software. The results are published in this paper. The challenge is now re-open as a online permanent challenge.Read more
Super-resolution fluorescence microscopy is an emerging multidisciplinary field that has opened up new opportunities in studying the living cell at the nanometer scale. It overcomes the classical ...Read more
This localization microscopy challenge goes hand in hand with the Open Bio Image Alliance (OBIA) mission. This consortium for bioimaging informatics aims at providing high-quality resources to bio ...Read more
The first dataset is released. The same sample is simulated in 4 modalities: 2D, 3D-astigmatism, 3D-biplane, and 3D-double-helix.
Released of the first experimental PSF, non-astigmatism, astigmatism, and double-helix.
Seamus Holden and Daniel Sage
Nature Photonics 10, 2016, PDF.
A new challenge will be held in August 2016. Send an email to be kept informed.
Advanced online publication of the comparative results on the Nature Methods website.
Update of the comprehensive list of SMLM software packages. 45 for localization software and 6 for deconvolution-type reconstruction software.
These reference datasets are designed to be largely used by the developpers to validate theirs software and by the users to check a software. They can be freely used if the sources and references are properly cited.
The ISBI Challenge is turned to a permanent online challenge.
Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 23 Mar 2016