Single-Molecule Localization Microscopy

Benchmarking of Single-Molecule Localization Microscopy Software

Comprehensive review of the single-molecule localization microscopy software based on reference datasets real datasets and synthetic datasets simulating biological structures and image formation process, and based on a objective assessment procedure.


This website presents the software for the reconstruction of the super-resolution images, in the context of single-molecule localization microscopy SMLM, PALM, or STORM.

The review is performed on the evaluation of the localization software on reference datassets using a well-defined metrics for the assessment.


Last Update

Online challenge 11 July 2013

The ISBI Challenge is turned to a permanent online challenge.

Software 01 July 2013

Update of the comprehensive list of localization and direct reconstruction software.

Conditions of use 01 May 2013

These reference datasets are designed to be largely used by the developpers to validate theirs software and by the users to check a software. They can be freely used if the sources and references are properly cited.

Familly of datasets 26 Apr. 2013

Usage 08 Apr. 2013

Some of these datasets have been used for the ISBI 2013 Grand Challenge organized in the conference IEEE ISBI 2013 at San Francisco to benchmark nearly 30 software.

© 2013 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 9 Aug 2013