Biomedical Imaging Group
Logo EPFL
    • Splines Tutorials
    • Splines Art Gallery
    • Wavelets Tutorials
    • Image denoising
    • ERC project: FUN-SP
    • Sparse Processes - Book Preview
    • ERC project: GlobalBioIm
    • The colored revolution of bioimaging
    • Deconvolution
    • SMLM
    • One-World Seminars: Representer theorems
    • A Unifying Representer Theorem
Follow us on Twitter.
Join our Github.
Masquer le formulaire de recherche
Menu
BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)
  1. School of Engineering STI
  2. Institute IEM
  3.  LIB
  4.  Steerable Filters
  • Laboratory
    • Laboratory
    • Laboratory
    • People
    • Jobs and Trainees
    • News
    • Events
    • Seminars
    • Resources (intranet)
    • Twitter
  • Research
    • Research
    • Researchs
    • Research Topics
    • Talks, Tutorials, and Reviews
  • Publications
    • Publications
    • Publications
    • Database of Publications
    • Talks, Tutorials, and Reviews
    • EPFL Infoscience
  • Code
    • Code
    • Code
    • Demos
    • Download Algorithms
    • Github
  • Teaching
    • Teaching
    • Teaching
    • Courses
    • Student projects
  • Splines
    • Teaching
    • Teaching
    • Splines Tutorials
    • Splines Art Gallery
    • Wavelets Tutorials
    • Image denoising
  • Sparsity
    • Teaching
    • Teaching
    • ERC project: FUN-SP
    • Sparse Processes - Book Preview
  • Imaging
    • Teaching
    • Teaching
    • ERC project: GlobalBioIm
    • The colored revolution of bioimaging
    • Deconvolution
    • SMLM
  • Machine Learning
    • Teaching
    • Teaching
    • One-World Seminars: Representer theorems
    • A Unifying Representer Theorem

Super-Resolution Orientation Estimation and Localization of Fluorescent Dipoles Using 3-D Steerable Filters

F. Aguet, S. Geissbühler, I. Märki, T. Lasser, M. Unser

Optics Express, vol. 17, no. 8, pp. 6829-6848, April 9, 2009.


Fluorophores that are fixed during image acquisition produce a diffraction pattern that is characteristic of the orientation of the fluorophore's underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by applying Gaussian-based fitting algorithms to in-focus images of individual fluorophores; when applied to fixed dipoles, this can lead to a bias in the range of 5-20 nm. We introduce a method for the joint estimation of position and orientation of dipoles, based on the representation of a physically realistic image formation model as a 3-D steerable filter. Our approach relies on a single, defocused acquisition. We establish theoretical, localization-based resolution limits on estimation accuracy using Cramér-Rao bounds, and experimentally show that estimation accuracies of at least 5 nm for position and of at least 2 degrees for orientation can be achieved. Patterns generated by applying the image formation model to estimated position/orientation pairs closely match experimental observations.

@ARTICLE(http://bigwww.epfl.ch/publications/aguet0901.html,
AUTHOR="Aguet, F. and Geissb{\"{u}}hler, S. and M{\"{a}}rki, I. and
	Lasser, T. and Unser, M.",
TITLE="Super-Resolution Orientation Estimation and Localization of
	Fluorescent Dipoles Using \mbox{3-D} Steerable Filters",
JOURNAL="Optics Express",
YEAR="2009",
volume="17",
number="8",
pages="6829--6848",
month="April 9,",
note="")

© 2009 OSA. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from OSA. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
  • Laboratory
  • Research
  • Publications
    • Database of Publications
    • Talks, Tutorials, and Reviews
    • EPFL Infoscience
  • Code
  • Teaching
Logo EPFL, Ecole polytechnique fédérale de Lausanne
Emergencies: +41 21 693 3000 Services and resources Contact Map Webmaster email

Follow EPFL on social media

Follow us on Facebook. Follow us on Twitter. Follow us on Instagram. Follow us on Youtube. Follow us on LinkedIn.
Accessibility Disclaimer Privacy policy

© 2023 EPFL, all rights reserved