EPFL
 Biomedical Imaging GroupSTI
EPFL
  Publications
English only   BIG > Publications > Analysis of Electropherograms


 CONTENTS
 Home Page
 News & Events
 People
 Publications
 Tutorials and Reviews
 Research
 Demos
 Download Algorithms

 DOWNLOAD
 PDF not available
 PS not available
 All BibTeX References

Computerized Methods for Analyzing Two-Dimensional Agarose Gel Electropherograms

A. Aldroubi, M. Unser, D. Tietz, B.L. Trus

Electrophoresis, vol. 12, no. 1, pp. 39-46, January 1991.



Previous methods interpret zonal or polydisperse gel patterns of two-dimensional Serwer-type gels in terms of size and free mobility (surface net charge density). These two parameters have been determined for each component without quantitatively measuring the abundance of the components. The present study advances these previous methods by determining the relative concentration of each component by computer evaluation of densitometrically analyzed gel patterns. Suitable procedures and their underlying algorithms are presented. The mathematical routines are implemented in a user-friendly software package, called GelFit and designed for a Macintosh personal computer. The program input consists of digitized images of gel staining patterns exemplified by those obtained from electrophoresis of native subcellular-sized particles. The data are processed through the following steps: (i) Noise reduction and calibration. (ii) Geometrical transformation of the pattern onto a rectangular size/free mobility coordinate system using rationales of the extended Ogston model. (iii) Analysis of the transformed image to determine density maxima, density profiles along iso-free-mobility or iso-size lines, curve fitting of one-dimensional profiles or two-dimensional surfaces using Gaussian functions and curve stripping of surfaces to determine the possible number of particle populations.


@ARTICLE(http://bigwww.epfl.ch/publications/aldroubi9101.html,
AUTHOR="Aldroubi, A. and Unser, M. and Tietz, D. and Trus, B.L.",
TITLE="Computerized Methods for Analyzing Two-Dimensional Agarose
        Gel Electropherograms",
JOURNAL="Electrophoresis",
YEAR="1991",
volume="12",
number="1",
pages="39--46",
month="January",
note="")

© 1991 Electrophoresis. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from Electrophoresis.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.