Imaging Neural Activity in the Ventral Nerve Cord of Behaving Adult Drosophila
C.-L. Chen, L. Hermans, M.C. Viswanathan, D. Fortun, F. Aymanns, M. Unser, A. Cammarato, M.H. Dickinson, P. Ramdya
Nature Communications, vol. 9, paper no. 4390, pp. 1-10, October 22, 2018.
To understand neural circuits that control limbs, one must measure their activity during behavior. Until now this goal has been challenging, because limb premotor and motor circuits have been largely inaccessible for large-scale recordings in intact, moving animals—a constraint that is true for both vertebrate and invertebrate models. Here, we introduce a method for 2-photon functional imaging from the ventral nerve cord (VNC) of behaving adult Drosophila melanogaster. We use this method to reveal patterns of activity across nerve cord populations during grooming and walking and to uncover the functional encoding of moonwalker ascending neurons (MANs), moonwalker descending neurons (MDNs), and a previously uncharacterized class of locomotion-associated A1 descending neurons. Finally, we develop a genetic reagent to destroy the indirect flight muscles and to facilitate experimental access to the VNC. Taken together, these approaches enable the direct investigation of circuits associated with complex limb movements.
AUTHOR="Chen, C.-L. and Hermans, L. and Viswanathan, M.C. and Fortun, D.
and Aymanns, F. and Unser, M. and Cammarato, A. and Dickinson, M.H.
and Ramdya, P.",
TITLE="Imaging Neural Activity in the Ventral Nerve Cord of Behaving
note="paper no.\ 4390")
Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.