EPFL
 Biomedical Imaging GroupSTI
EPFL
  Publications
English only   BIG > Publications > Cellular Ultrastructures


 CONTENTS
 Home Page
 News & Events
 People
 Publications
 Tutorials and Reviews
 Research
 Demos
 Download Algorithms

 DOWNLOAD
 PDF
 Postscript
 All BibTeX References

Imaging Cellular Ultrastructures Using Expansion Microscopy (U-ExM)

D. Gambarotto, F.U. Zwettler, M. Le Guennec, M. Schmidt-Cernohorska, D. Fortun, S. Borgers, J. Heine, J.-G. Schloetel, M. Reuss, M. Unser, E.S. Boyden, M. Sauer, V. Hamel, P. Guichard

Nature Methods—Techniques for Life Scientists and Chemists, vol. 16, no. 1, pp. 71-81, January 2019.



Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.


@ARTICLE(http://bigwww.epfl.ch/publications/gambarotto1901.html,
AUTHOR="Gambarotto, D. and Zwettler, F.U. and Le Guennec, M. and
        Schmidt-Cernohorska, M. and Fortun, D. and Borgers, S. and Heine, J.
        and Schloetel, J.-G. and Reuss, M. and Unser, M. and Boyden, E.S.
        and Sauer, M. and Hamel, V. and Guichard, P.",
TITLE="Imaging Cellular Ultrastructures Using Expansion Microscopy
        ({U-ExM})",
JOURNAL="Nature Methods---Techniques for Life Scientists and Chemists",
YEAR="2019",
volume="16",
number="1",
pages="71--81",
month="January",
note="")

© 2019 The Authors CC-BY. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from The Authors CC-BY.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.