Imaging Cellular Ultrastructures Using Expansion Microscopy (U-ExM)
D. Gambarotto, F.U. Zwettler, M. Le Guennec, M. Schmidt-Cernohorska, D. Fortun, S. Borgers, J. Heine, J.-G. Schloetel, M. Reuss, M. Unser, E.S. Boyden, M. Sauer, V. Hamel, P. Guichard
Nature Methods—Techniques for Life Scientists and Chemists, vol. 16, no. 1, pp. 71-81, January 2019.
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.
AUTHOR="Gambarotto, D. and Zwettler, F.U. and Le Guennec, M. and
Schmidt-Cernohorska, M. and Fortun, D. and Borgers, S. and Heine, J.
and Schloetel, J.-G. and Reuss, M. and Unser, M. and Boyden, E.S.
and Sauer, M. and Hamel, V. and Guichard, P.",
TITLE="Imaging Cellular Ultrastructures Using Expansion Microscopy
JOURNAL="Nature Methods---Techniques for Life Scientists and Chemists",
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