Biomedical Imaging GroupSTI
English only   BIG > Publications > SMLM Workshop

 Home Page
 News & Events
 Tutorials and Reviews
 Download Algorithms

 All BibTeX References

SMLM Flight Simulator Workshop

J. Griffié, D. Sage

Proceedings of the 2020 Quantitative BioImaging Conference (QBI'20), Oxford, United Kingdom, January 6-9, 2020, paper no. 536.

Super resolution has enabled the study of the cellular architecture under the diffraction limit of light for the first time, while retaining the advantages of conventional fluorescence microscopy (e.g. staining specificity). Although, single molecule localisation microscopy (SMLM) leads to the biggest gain in spatial resolution (typically 10-30nm), it remains paradoxically rarely used as a routine tool in biology laboratories, and overall, suffers from the lack of reproducibility of the produced images. Both the complexity of the image acquisition with many user-defined parameters to set (e.g. intensity of the excitation/activation laser, frame rate), and the lack of guide lines to do so, contribute to these limitations. We will present during this workshop a novel easy-to-use simulation platform, or SMLM flight simulator, that aims at tackling both issues. This simulator runs in real-time allowing effective interactivity. It generates hundreds of images from a 3D structure defined by hundreds of thousands of points. Our flight simulator consists of a virtual microscope set up that recreates the environment of SMLM acquisition setup. The platform is designed for instance for the training of new users in producing reliable SMLM data sets. It also allows for the validation of new quantitative methods, as well as enables experienced users to fully characterised the feasibility of future experiments.

AUTHOR="Griffi{\'{e}}, J. and Sage, D.",
TITLE="{SMLM} Flight Simulator Workshop",
BOOKTITLE="Proceedings of the 2020 Quantitative BioImaging Conference
address="Oxford, United Kingdom",
month="January 6-9,",
note="paper no.\ 536")

© 2020 QBI. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from QBI.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.