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SMLM Flight Simulator Workshop

J. Griffié, D. Sage

Proceedings of the 2020 Quantitative BioImaging Conference (QBI'20), Oxford, United Kingdom, January 6-9, 2020, paper no. 536.



Super resolution has enabled the study of the cellular architecture under the diffraction limit of light for the first time, while retaining the advantages of conventional fluorescence microscopy (e.g. staining specificity). Although, single molecule localisation microscopy (SMLM) leads to the biggest gain in spatial resolution (typically 10-30nm), it remains paradoxically rarely used as a routine tool in biology laboratories, and overall, suffers from the lack of reproducibility of the produced images. Both the complexity of the image acquisition with many user-defined parameters to set (e.g. intensity of the excitation/activation laser, frame rate), and the lack of guide lines to do so, contribute to these limitations. We will present during this workshop a novel easy-to-use simulation platform, or SMLM flight simulator, that aims at tackling both issues. This simulator runs in real-time allowing effective interactivity. It generates hundreds of images from a 3D structure defined by hundreds of thousands of points. Our flight simulator consists of a virtual microscope set up that recreates the environment of SMLM acquisition setup. The platform is designed for instance for the training of new users in producing reliable SMLM data sets. It also allows for the validation of new quantitative methods, as well as enables experienced users to fully characterised the feasibility of future experiments.


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