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A Guided Tour of Selected Image Processing and Analysis Methods for Fluorescence and Electron Microscopy

C. Kervrann, C.Ó.S. Sorzano, S.T. Acton, J.-C. Olivo-Marin, M. Unser

IEEE Journal of Selected Topics in Signal Processing, vol. 10, no. 1, pp. 6-30, February 2016.


Microscopy imaging, including fluorescence microscopy and electron microscopy, has taken a prominent role in life science research and medicine due to its ability to investigate the 3D interior of live cells and organisms. A long-term research in bio-imaging at the sub-cellular and cellular scales consists then in inferring the relationships between the dynamics of macromolecules and their functions. In this area, image processing and analysis methods are now essential to understand the dynamic organization of groups of interacting molecules inside molecular machineries and to address issues in fundamental biology driven by advances in molecular biology, optics and technology. In this paper, we present recent advances in fluorescence and electron microscopy and we focus on dedicated image processing and analysis methods required to quantify phenotypes for a limited number but typical studies in cell imaging.

@ARTICLE(http://bigwww.epfl.ch/publications/kervrann1601.html,
AUTHOR="Kervrann, C. and Sorzano, C.{\'{O}}.S. and Acton, S.T. and
	Olivo-Marin, J.-C. and Unser, M.",
TITLE="A Guided Tour of Selected Image Processing and Analysis Methods
	for Fluorescence and Electron Microscopy",
JOURNAL="{IEEE} Journal of Selected Topics in Signal Processing",
YEAR="2016",
volume="10",
number="1",
pages="6--30",
month="February",
note="")

© 2016 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from IEEE. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
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