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Can Localization Microscopy Benefit from Approximation Theory?

H. Kirshner, C. Vonesch, M. Unser

Proceedings of the Tenth IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI'13), San Francisco CA, USA, April 7-11, 2013, pp. 584-587.


We introduce a general and computationally efficient approach to 3-D localization microscopy. The main idea is to construct a continuous-domain representation of the PSF by expanding it in a polynomial B-spline basis. This allows us to fit the PSF to the data with sub-pixel accuracy. Since the basis functions are compactly supported, the evaluation of the PSF is computationally efficient. Furthermore, our approach can accommodate for any 3-D PSF design, and it does not require a calibration curve for the axial position. We further introduce a computationally efficient implementation of the least-squares criterion and demonstrate its potential use for fast and accurate reconstruction of super-resolution data.

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AUTHOR="Kirshner, H. and Vonesch, C. and Unser, M.",
TITLE="Can Localization Microscopy Benefit from Approximation Theory?",
BOOKTITLE="Proceedings of the Tenth IEEE International Symposium on
	Biomedical Imaging: {F}rom Nano to Macro ({ISBI'13})",
YEAR="2013",
editor="",
volume="",
series="",
pages="584--587",
address="San Francisco CA, USA",
month="April 7-11,",
organization="",
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