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Automated Tracking of Single Fluorescence Particle

D. Sage, M. Unser

Proceedings of the 2004 Annual Meeting of the Swiss Society of Biomedical Engineering (SSBE'04), Zürich ZH, Swiss Confederation, September 2-3, 2004, poster 55.



We present a novel and robust computational procedure for tracking fluorescent particles in images of time-lapse microscopy. The algorithm is optimized for finding the trajectory of single particles in very noisy dynamic image sequences. We have applied the software to trace the movement of chromosoma ltelomeres within the nucleus of a yeast cell [1]. The new algorithm reduces the analysis time of a 300 images sequence from 10 minutes, when it is done manually, to just a few seconds. It also offers the benefit of reproducibility.

References

  1. S.M. Gasser, "Positions of Potential: Nuclear Organization and Gene Expression," Cell, vol. 104, no. 5, pp. 639-642, March 2001.


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