3D Deconvolution Microscopy
F. Soulez, D. Sage
Proceedings of Functional Microscopy for Biology (MiFoBio'18), Seignosses, French Republic, October 5-12, 2018, pp. 113.
Le but de la déconvolution est de compenser numériquement le flou introduit par le microscope. En microscopie 3D, la déconvolution permet de restaurer les images 3D notamment:
- en améliorant la résolution (axiale en particulier),
- en réduisant le bruit (en particulier à faible flux),
- en augmentant le contraste.
Cela fait de la déconvolution un outil précieux pour rendre possible la segmentation et la quantification des images.
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