Spatial Disorders and Computational Cures
A.C. Steven, E. Kocsis, M. Unser, B.L. Trus
International Journal of Biological Macromolecules, vol. 13, no. 3, pp. 174–180, June 1991.
Image averaging provides a powerful method for enhancing the yield of interpretable information from electron micrographs of biological macromolecules. However, as originally conceived, the full benefit of averaging is achieved only with perfectly ordered two-dimensional crystals. More recent developments, reviewed here, allow one to rectify disordered lattices, straighten randomly bent filaments, and combine multiple images of free-standing particles, thus extending the advantages of image averaging to virtually every class of macromolecular specimen.
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