Image Processing and Analysis of Biological Images

M. Unser, D. Sage, M. Jacob, E. Meijering, P. Thévenaz

Live Cell Imaging Workshop, Swiss Federal Institute of Technology Lausanne, Switzerland, October 9-10, 2002.

We will provide an overview of our research efforts relating to the processing and analysis of biological images. In particular, we will present a novel particle tracking algorithm and show some preliminary results on fluorescence microscopy images. We will also describe a detector of filament-like structures that outperforms the classical differential approaches (gradient or Hessian-based). Another most relevant development is a graph search technique that permits an efficient, semi-automatic tracking of dendrites in neurons. Finally, we will discuss our spline-based approach to image registration that is well suited for the alignment of time-lapse images (2D and 3D), or for creating mosaics. Most of our algorithms are available as JAVA plug-ins for the public domain ImageJ software, which was developed by Wayne Rasband from the NIH.

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