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Determination of Rules Governing Spindle Pole Asymmetry in SIN Signalling

P. Wachowicz, D. Schmitter, D. Sage, M. Unser, L. Cerutti, I. Xenarios, V. Simanis

EMBO Conference Series on Centrosomes & Spindle Pole Bodies (CSPD'11), Barcelona, Kingdom of Spain, October 2-6, 2011.



In Schizosaccaromyces pombe, cytokinesis initiation and progression is governed by a group of protein kinases called the Septation Initiation Network (SIN). These proteins are recruited and progressively activated on spindle pole bodies (SPB). Interestingly, although in initial mitotic phases some SIN proteins are distributed symmetrically on both SPBs, after sister chromatid separation they become enriched on only one SPB, while fading on the other. This leads to SPB asymmetry at the end of mitosis, which may be preserved after cell division.

It is known that the SIN activity is regulated by nucleotide status of the signaling GTPase, spg1p, whose nucleotide status is controlled by the GAP, cdc16p-byr4p and a putative GEF, etd1p. We are combining automated image analysis and in silico modeling to determine the rules governing the transition of SIN proteins from the symmetric to asymmetric state, which correlates with activation of the SIN. We also plan to extend this analysis to mutant cells, to study the consequences of interfering with this control circuitry.


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