|Tracing of Neurites|
Investigators: Erik Meijering, Mathews Jacob
We have developed an algorithm to facilitate the tracing and quantitation of neurites in two-dimensional (2D) fluorescence microscopy images. The tracing is done interactively based on the specification of end points; the optimal path is determined on the fly from the optimization of a cost function using Dijkstra's shortest-path algorithm. The procedure also takes advantage of an improved ridge detector implemented by means of a steerable filterbank.
The software is available as a Java pluging,
NeuronJ, for ImageJ.
Collaborations: Prof. Michael Unser; Dr. Harard Hirling, Juan-Carlos Sarria, Pascal Steiner (Institute of Neurosciences, EPFL)
E. Meijering, M. Jacob, J.-C.F. Sarria, P. Steiner, H. Hirling, M. Unser, "Design and Validation of a Tool for Neurite Tracing and Analysis in Fluorescence Microscopy Images," Cytometry Part A, vol. 58A, no. 2, pp. 167-176, April 2004.
© 2013 EPFL • • 16.12.2013