An ImageJ plugin for tracking single particle over noisy images sequence
A moving particle in a cell nucleus
Path in red over one specific XY image
Path in red over X(t) maximum intensity projection
DescriptionSpotTracker is a robust and fast computational procedure for tracking fluorescent markers in time-lapse microscopy. The algorithm is optimized for finding the time-trajectory of single particles in very noisy image sequences. The optimal trajectory of the particle is extracted by applying a dynamic programming optimization procedure. In addition, SpotTracker includes a optimal filter to enhance a Gaussian-like spot while attenuating the background noise.
D. Sage, F.R. Neumann, F. Hediger, S.M. Gasser, M. Unser, "Automatic Tracking of Individual Fluorescence Particles: Application to the Study of Chromosome Dynamics," IEEE Transactions on Image Processing, vol. 14, no. 9, pp. 1372-1383, September 2005.
The software provided here is a plugin for ImageJ, a general purpose image-processing and image-analysis package. ImageJ has a public domain licence; it runs on several plateforms: Unix, Linux, Windows, Mac OS 9 and Mac OS X. It doesn't take more than a couple of minutes to install.
Download and install
These plugins consist in one single JAR file; place it into the "plugins" folder of ImageJ. Do not unzip the JAR file.
It exists three versions:
A image sequence is available at: Sequence2D.zip (418 Kb).
Conditions of use
You'll be free to use this software for research purposes, but you must not transmit and distribute it without our consent. In addition, you undertake to include a citation whenever you present or publish results that are based on it. EPFL makes no warranties of any kind on this software and shall in no event be liable for damages of any kind in connection with the use and exploitation of this technology.