Single-Molecule Localization Microscopy  •  Software Benchmarking

2 LS

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sequence.zip [257 Mb]

Acquisition Parameters

Camera
Photon converter factor or Quantum efficiency (QE)1.00e-/Ph.
QE = QE-Gain x QE-interacting [Ref. 1]
Resolution128pixels
Pixelsize150.00nm
Field of view19200.00nm
Optics
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Drift
DeriveDisable
ShakingDisable
Analog Digital Conversion
Electron conversion - Gain2.00DN/e-
Electron conversion - Offset0.00DN
Baseline100.00DN
Saturation16383.00DN
Quantization14-bit

Reference

[1] James R. Janesick, Photon Transfer, SPIE Press Monograph Vol. PM170, 2007.

Point-Spread Function (PSF)

Physical Model
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Offset focal plane 0nm
ModelGibson and Lanni
Refractive index sample1.00
Refractive index immersion1.50
Offest working distance0.00nm

Reference

[1] H. Kirshner, F. Aguet, D. Sage, M. Unser, "3-D PSF Fitting for Fluorescence Microscopy: Implementation and Localization Applications", Journal of Microscopy, in press.

[2] PSF Generator An ImageJ plugin to generate 3D microscope Point-Spread Function (PSF).

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018