Single-Molecule Localization Microscopy  •  Software Benchmarking

Bundled Tubes High Density

Updated 02 February 2013

Bundles of 8 tubes of 30 nm diameter
High-density, 81049 fluorophores on 168 frames

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Sequence Download all frames
sequence.zip [2028 Kb]

Acquisition Parameters

Camera
Photon converter factor or Quantum efficiency (QE)1.00e-/Ph.
QE = QE-Gain x QE-interacting [Ref. 1]
Resolution64pixels
Pixelsize100.00nm
Field of view6400.00nm
Optics
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Drift
DeriveDisable
ShakingDisable
Analog Digital Conversion
Electron conversion - Gain1.00DN/e-
Electron conversion - Offset0.00DN
Baseline100.00DN
Saturation16383.00DN
Quantization14-bit

Reference

[1] James R. Janesick, Photon Transfer, SPIE Press Monograph Vol. PM170, 2007.

Point-Spread Function (PSF)

Physical Model
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Offset focal plane 0nm
XY functionGaussian
FWHM258.21nm
Z functionExponential
Focal plane1 x FWHM at 0.0 nm
Defocus plane2 x FWHM at 300.0 nm

Reference

[1] H. Kirshner, F. Aguet, D. Sage, M. Unser, "3-D PSF Fitting for Fluorescence Microscopy: Implementation and Localization Applications", Journal of Microscopy, in press.

[2] PSF Generator An ImageJ plugin to generate 3D microscope Point-Spread Function (PSF).

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Last update: 31 Mar 2017