Single-Molecule Localization Microscopy  •  Software Benchmarking

The Challenge 2013 is turned to an online permanent challenge

The Grand Challenge Localization Microscopy was organized as Workshop in the conference IEEE International Symposium on Biomedical Imaging. Nearly 30 software have been run, mostly by the authors of the software, thus constituting the first world-wide effort in benchmarking the localization software in a comprehensive review. The results are now published.

The 2013 ISBI challenge is now re-open as a online permanent challenge and a new SMLM challenge will be held in 2016.

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Realistic Simulations • Challenge 2013

These datasets are simulated sequence of realistic frames. The bio-inspired samples are 3D continuous models that imitates biological structures. These datasets were used for the challenge 2013.

Tubulins_I 2D

Published February 1, 2013 as training dataset for the challenge 2013

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This sample consists to a realistic structure of 7 tubulins (constant diameter 25 nm) and 1 tubulin (constant diameter 40 nm).
The depth of the sample is from 0 to 300 nm.
100000 fluorophores are activated over 2400 frames.
Low level of read-out noise autofluorescence background.

Tubulins_II 2D

Published February 1, 2013 as training dataset for the challenge 2013

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This sample consists to a realistic structure of 7 tubulins (constant diameter 25 nm) and 1 tubulin (constant diameter 40 nm).
The depth of the sample is from 0 to 300 nm.
100000 fluorophores are activated over 2400 frames.
High level of read-out noise and autofluorescence background.

Bundled_Tubes_Long_Sequence 2D

Published February 2, 2013 as training dataset for the challenge 2013

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Bundles of 8 tubes of 30 nm diameter
Sparse density, 81049 molecules on 12000 frames

Bundled_Tubes_High_Density 2D

Published February 2, 2013 as training dataset for the challenge 2013

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Bundles of 8 tubes of 30 nm diameter
High-density, 81049 fluorophores on 168 frames

Contest Dataset 1 • Low Density (LS) 2D

Published February 2, 2013 as contest dataset for the challenge 2013

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Tubulins of various diameters
10'000 frames

Contest Dataset 1 • High Density (HD) 2D

Published February 2, 2013 as contest dataset for the challenge 2013

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Tubulins of various diameters
1'000 frames • high-density of fluorophores

Contest Dataset 2 • Low Density (LS) 2D

Published February 2, 2013 as contest dataset for the challenge 2013

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Network of tubulins
12000 frames

Contest Dataset 2 • High Density (HD) 2D

Published February 2, 2013 as contest dataset for the challenge 2013

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Network of tubulins
204 frames • high-density of fluorophores

Contest Dataset 3 • Low Density (HD) 2D

Published February 2, 2013 as contest dataset for the challenge 2013

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Helicoidal tubes, deep sample from 0 to 1μm
7000 frames

Contest Dataset 3 • High Density (HD) 2D

Published February 2, 2013 as contest dataset for the challenge 2013

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Helicoidal tubes, deep sample from 0 to 1μm
600 frames • high-density of fluorophores

© 2017 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017