Single-Molecule Localization Microscopy  •  Software Benchmarking

Challenge 2D • Theoretical PSF

The Grand Challenge Localization Microscopy was presented as a Workshop in IEEE conference ISBI. Nearly 30 software have been run, mostly by the authors of the software, thus constituting the first world-wide effort in benchmarking the localization software in a comprehensive review.

The results are in the paper: Sage et al. Quantitative evaluation of software packages for SMLM, Nature Methods 12, 2015.


IEEE ISBI Workshop

April, 8, 2013, San Francisco
Presentation Speaker
IntroductionDaniel Sage
PSF models Hagai Kirshner
Photon quantification Nico Stuurman
Datasets Daniel Sage
Rendering Thomas Pengo
File format Nico Stuurman
Assessment Daniel Sage
B-recsHerveé Raoult
FacePALM Eelco Hoogendoorn
FALCON Junhong Min
Fast-ML-HD Kyungsang Kim
FPGA Manfred Kirchgessner
GPUgaussMLE Keith Lidke
Insight3Ryan McGorty
MicroManager LM Nico Stuurman
QuickPALM Christophe Zimmer
RadialSymmetry Raghuveer Parthasarathy
simpleSTORM Luca Fiaschi
SOSpluginIhor Smal
ThunderSTORMPavel Krizek
Wavelet FluoroBrancroft Trevor Ashley
Additional software (a-livePALM, DOASTORM, PeakFit, RapidSTORM)Thomas Pengo
Results - Real datasets Junhong Min
Results - Localization Thomas Pengo
Round-table, discussions Everybody

Be involved! the Challenge 2013 is turned to an permanent challenge!

To announce your participation, just send an email to Daniel Sage indicating the name of the software that you want to represent or to test and the name of a contact person.

Access to the datasets

Pre-registration gives access to the ground-truth of the training datasets and to the frame sequence of the contest datasets.

Open to everybody

The challenge is open to all individuals or teams, academic or corporate, existing or newly developed localization microscopy software. Participation is free.


Pre-registration will create your own account to submit your localization results.

Challenge discussion forum

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Last update: 30 Nov 2018