Single-Molecule Localization Microscopy  •  Software Benchmarking

The Challenge 2013 is turned to an online permanent challenge

The Grand Challenge Localization Microscopy was organized as Workshop in the conference IEEE International Symposium on Biomedical Imaging. Nearly 30 software have been run, mostly by the authors of the software, thus constituting the first world-wide effort in benchmarking the localization software in a comprehensive review. The results are now published.

The 2013 ISBI challenge is now re-open as a online permanent challenge and a new SMLM challenge will be held in 2016.


IEEE ISBI Workshop

April, 8, 2013, San Francisco
Presentation Speaker
IntroductionDaniel Sage
PSF models Hagai Kirshner
Photon quantification Nico Stuurman
Datasets Daniel Sage
Rendering Thomas Pengo
File format Nico Stuurman
Assessment Daniel Sage
B-recsHerveé Raoult
FacePALM Eelco Hoogendoorn
FALCON Junhong Min
Fast-ML-HD Kyungsang Kim
FPGA Manfred Kirchgessner
GPUgaussMLE Keith Lidke
Insight3Ryan McGorty
MicroManager LM Nico Stuurman
QuickPALM Christophe Zimmer
RadialSymmetry Raghuveer Parthasarathy
simpleSTORM Luca Fiaschi
SOSpluginIhor Smal
ThunderSTORMPavel Krizek
Wavelet FluoroBrancroft Trevor Ashley
Additional software (a-livePALM, DOASTORM, PeakFit, RapidSTORM)Thomas Pengo
Results - Real datasets Junhong Min
Results - Localization Thomas Pengo
Round-table, discussions Everybody

Be involved! the Challenge 2013 is turned to an permanent challenge!

To announce your participation, just send an email to Daniel Sage indicating the name of the software that you want to represent or to test and the name of a contact person.

Access to the datasets

Pre-registration gives access to the ground-truth of the training datasets and to the frame sequence of the contest datasets.

Open to everybody

The challenge is open to all individuals or teams, academic or corporate, existing or newly developed localization microscopy software. Participation is free.


Pre-registration will create your own account to submit your localization results.

Challenge discussion forum

© 2017 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017