Single-Molecule Localization Microscopy  •  Software Benchmarking

Results • Beads

Experimental conditions
  • z-stack of 6 beads in 2D, 3D-Astigmatism, 3D-Double-Helix, 3D-Biplane
  • Z-step = 10nm, Z-range = -750nm to 750nm, Z-focal plane at 0nm (151 slices)

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Results

BaselineLocalization_Astigmatism.csv

Tolerance XYTolerance ZReference# fluosTest# fluosEvaluationWoobleMin. Photons in RefIntersectionJaccardPrecisionRecallRMSE LateralRMSE AxialDeltaXDeltaYDeltaZmd Intensity
320.0320.0Ground-truth906Tested1017XYZ (cylinder)NO0.022012.9180.2160.24361.148182.19732.613-47.15729.172-22833.445
320.0320.0Ground-truth906Tested1017XY (disk)NO0.080672.1580.7930.89099.90616.948-43.572-25116.839

© 2017 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017