Single-Molecule Localization Microscopy  •  Software Benchmarking

Data • ER2.N3.HD

Experimental conditions
  • Structure: simulation of cellular organelle (endoplasmic reticulum/mitochondria) inspired structure in the field of view of 6.4 x 6.4 x 0.7 μm
  • Sequence: 3'020 frames
  • Modality: 2D
  • Noise N3: typical photon counts and level of background for site specific dye-labelled live cell STORM sample such as ER Tracker
  • Molecule density: 5

banner

Download

Type Modality Link to Download Format Size
Sequence2DER2.N3.HD-2D-Exp-as-listZIP, n TIFF images20 Mb
Sequence2DER2.N3.HD-2D-Exp-as-stackZIP, 1 TIFF stack of n images19 Mb

Parameters

Camera
Photon converter factor or Quantum efficiency (QE)0.90e-/Ph.
Resolution64pixels
Pixelsize100.00nm
Field of view6400.00nm
Optics
Wavelength660.00nm
Numerical aperture (NA)1.49
Autofluorescence
Poisson Distribution
Camera Noise
Read-out: Gaussian distribution74.4
EMGain: Gamma300.0
Spurious noise: Poisson distribution0.0020
Analog Digital Conversion
Total gain: QE*EM_gain/e_per_ADU6.00
Electron conversion e- per ADU45.00e-/DN
Baseline100.00DN
Saturation65535.00DN
Quantization16-bit
Computational Parameters
Thickness1500.00nm
Frames19620
MultithreadingOff-1 Thread
Pixelsize to PSF convolution10.0nm
Pixelsize for autofluorescence100.0nm
Pixelsize of the camera100.0nm
File formatTIFF 16-bits

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018