Single-Molecule Localization Microscopy  •  Software Benchmarking

Oracle-2D • MT0.N1.HD

Experimental conditions
  • Structure: MT0, 3 microtubules in the field of view of 6.4 x 6.4 x 1.5 μm
  • Sequence: 2'500 frames
  • Modality: 2D, 3D-Astigmatism, 3D-Double-Helix, 3D-Biplane
  • Noise N1: typical photon counts and background levels for Alexa647 labelled STORM sample
  • Molecule density: 2

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Sample

Activation

feature,value 
Number of fluos,  11172
Number of frames,   2500
Field of view (nm), 6400.00
Number of activations,  31612
Average number of photons / activation, 4621.477658
Density - number of fluorophores per frame, 12.644800
Density - number of photons per frame, 58437.660687
Center of power illumination X (nm), 2948.519
Center of power illumination X (nm), 3473.742
Distance at half maximum illumination (nm), 3520.000
Distance at quarter maximum illumination (nm), 4480.000
Power illumination (W), 400.000
Framerate, 50.000
Maximum flux of photons per frame, 7852.507
Threshold for activation (min photons), 78.525
Ton,  3.000
Tdark,  2.500
Tbleaching,  1.500

Excerpt

First Frame: 00001.tif

Random Frame: 00528.tif

Random Frame: 00522.tif

Last Frame: 02500.tif

Time projection

Average Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Maximum Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Report of simulation

FeatureCountMinMaxMeanStdev
ID316121.00031612.00015806.500513.270
X316121.7936398.7642927.19193.241
Y316120.8376398.5473032.84993.944
Z31612-667.568634.80858.5517.258
Frame316121.0002500.0001259.59340.832
Photons3161278.57210578.7394621.478157.198
Channel316120.0000.0000.0000.000
Frame ON316120.0000.0000.0000.000
Total316121.0001.0001.0000.028
Background Mean31612327.5832619.688991.44129.074
Background Stdev3161227.9681935.328195.4847.253
Signal Mean31612279.4007352.6801814.87556.553
Signal Stdev316123.8112172.824252.1119.410
Signal Peak31612774.00013312.0003165.644102.767
Sigma X316120.0000.0000.0000.000
Sigma Y316120.0000.0000.0000.000
Sigma Z316120.0000.0000.0000.000
Uncertainty316120.0000.0000.0000.000
Closest ID316120.00031605.0008138.864367.747
Closest Distance316122.673442.953334.13510.191
Closest Count316121.0006.0001.2830.039
CNR31612-1.075106.13414.8190.538
SNR31612-1.07139.5376.7390.211
PSNR316120.998125.18322.5970.752
Unknown316120.0000.0000.0000.000

© 2017 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017