Single-Molecule Localization Microscopy  •  Software Benchmarking

Oracle-BP • MT0.N1.LD

Experimental conditions
  • Structure: MT0, 3 microtubules in the field of view of 6.4 x 6.4 x 1.5 μm
  • Sequence: 19'996 frames
  • Modality: 2D, 3D-Astigmatism, 3D-Double-Helix, 3D-Biplane
  • Noise N1: typical photon counts and background levels for Alexa647 labelled STORM sample
  • Molecule density: 0.2

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Sample

Activation

feature,value 
Number of fluos,   8731
Number of frames,  20000
Field of view (nm), 6400.00
Number of activations,  24938
Average number of photons / activation, 4604.954740
Density - number of fluorophores per frame, 1.246900
Density - number of photons per frame, 5741.918065
Center of power illumination X (nm), 2948.519
Center of power illumination X (nm), 3473.742
Distance at half maximum illumination (nm), 3520.000
Distance at quarter maximum illumination (nm), 4480.000
Power illumination (W), 400.000
Framerate, 50.000
Maximum flux of photons per frame, 7852.507
Threshold for activation (min photons), 78.525
Ton,  3.000
Tdark,  2.500
Tbleaching,  1.500

Excerpt

First Frame: 00001.tif

Random Frame: 15986.tif

Random Frame: 02628.tif

Last Frame: 19996.tif

Time projection

Average Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Maximum Intensity Projection

Camera Resolution: 100.0nm/pixel (Original size)

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High Resolution: 10.0nm/pixel (Enlarge this image)

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Report of simulation

FeatureCountMinMaxMeanStdev
ID249381.00024938.00012469.500455.883
X249382.0886398.7642935.322105.270
Y249383.1686398.5473018.019105.324
Z24938-667.568634.80857.1018.235
Frame249381.00019996.00010038.922366.656
Photons2493878.78310576.8604604.955176.342
Channel249380.0000.0000.0000.000
Frame ON249380.0000.0000.0000.000
Total249381.0001.0001.0000.032
Background Mean24938322.4031140.889727.38323.348
Background Stdev2493824.723600.38674.3882.804
Signal Mean24938262.2003112.000981.08232.364
Signal Stdev249382.291914.77077.5703.332
Signal Peak24938759.0006885.0001394.09347.779
Sigma X249380.0000.0000.0000.000
Sigma Y249380.0000.0000.0000.000
Sigma Z249380.0000.0000.0000.000
Uncertainty249380.0000.0000.0000.000
Closest ID249380.00024928.000893.584121.896
Closest Distance249387.560442.953429.40513.726
Closest Count249381.0003.0001.0280.033
CNR24938-0.00277.01810.0880.407
SNR24938-0.00223.5945.9170.206
PSNR249381.87596.37922.8950.792
Unknown249380.0000.0000.0000.000

© 2017 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017