Single-Molecule Localization Microscopy  •  Software Benchmarking

Data • MT1.N1.LD

Experimental conditions
  • Structure: MT1, 3 microtubules in the field of view of 6.4 x 6.4 x 1.5 μm
  • Sequence: 19'996 frames
  • Modality: 3D-Astigmatism, 3D-Double-Helix, 3D-Biplane
  • Noise N1: typical photon counts and background levels for Alexa647 labelled STORM sample
  • Molecule density: 0.2

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Download

Type Modality Link to Download Format Size
Sequence3D-AstigmatismMT1.N1.LD-AS-Exp-as-listZIP, n TIFF images122 Mb
Sequence3D-AstigmatismMT1.N1.LD-AS-Exp-as-stackZIP, 1 TIFF stack of n images116 Mb
Sequence3D-Biplane (1 plane +250)MT1.N1.LD-BP+250-as-listZIP, n TIFF images121 Mb
Sequence3D-Biplane (1 plane +250)MT1.N1.LD-BP+250-as-stackZIP, 1 TIFF stack of n images115 Mb
Sequence3D-Biplane (1 plane -250)MT1.N1.LD-BP-250-as-listZIP, n TIFF images121 Mb
Sequence3D-Biplane (1 plane -250)MT1.N1.LD-BP-250-as-stackZIP, 1 TIFF stack of n images115 Mb
Sequence3D-Biplane (2 planes)MT1.N1.LD-BP-Exp-as-listZIP, n TIFF images237 Mb
Sequence3D-Biplane (2 planes)MT1.N1.LD-BP-Exp-as-stackZIP, 1 TIFF stack of n images230 Mb
Sequence3D-Double-HelixMT1.N1.LD-DH-Exp-as-listZIP, n TIFF images124 Mb
Sequence3D-Double-HelixMT1.N1.LD-DH-Exp-as-stackZIP, 1 TIFF stack of n images116 Mb

Parameters

Camera
Photon converter factor or Quantum efficiency (QE)0.90e-/Ph.
Resolution64pixels
Pixelsize100.00nm
Field of view6400.00nm
Optics
Wavelength660.00nm
Numerical aperture (NA)1.49
Autofluorescence
Poisson Distribution
Camera Noise
Read-out: Gaussian distribution74.4
EMGain: Gamma300.0
Spurious noise: Poisson distribution0.0020
Analog Digital Conversion
Total gain: QE*EM_gain/e_per_ADU6.00
Electron conversion e- per ADU45.00e-/DN
Baseline100.00DN
Saturation65535.00DN
Quantization16-bit
Computational Parameters
Thickness1500.00nm
Frames19996
MultithreadingOff-1 Thread
Pixelsize to PSF convolution10.0nm
Pixelsize for autofluorescence100.0nm
Pixelsize of the camera100.0nm
File formatTIFF 16-bits

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018