Single-Molecule Localization Microscopy  •  Software Benchmarking

Data • MT4.N2.HD

Experimental conditions
  • Structure: 3 microtubules in the field of view of 6.4 x 6.4 x 1.5 μm
  • Modality: 2D, 3D-Astigmatism, 3D-Double-Helix, 3D-Biplane
  • Noise N2: photoswitchable fluorescent protein labelled sample such as mEos2 or Dendra2

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Download

Type Modality Link to Download Format Size
Sequence2DMT4.N2.HD-2D-Exp-as-listZIP, n TIFF images16 Mb
Sequence2DMT4.N2.HD-2D-Exp-as-stackZIP, 1 TIFF stack of n images14 Mb
Sequence3D-AstigmatismMT4.N2.HD-AS-Exp-as-listZIP, n TIFF images16 Mb
Sequence3D-AstigmatismMT4.N2.HD-AS-Exp-as-stackZIP, 1 TIFF stack of n images14 Mb
Sequence3D-Biplane (1 plane +250)MT4.N2.HD-BP+250-as-listZIP, n TIFF images16 Mb
Sequence3D-Biplane (1 plane +250)MT4.N2.HD-BP+250-as-stackZIP, 1 TIFF stack of n images14 Mb
Sequence3D-Biplane (1 plane -250)MT4.N2.HD-BP-250-as-listZIP, n TIFF images16 Mb
Sequence3D-Biplane (1 plane -250)MT4.N2.HD-BP-250-as-stackZIP, 1 TIFF stack of n images14 Mb
Sequence3D-Biplane (2 planes)MT4.N2.HD-BP-Exp-as-listZIP, n TIFF images30 Mb
Sequence3D-Biplane (2 planes)MT4.N2.HD-BP-Exp-as-stackZIP, 1 TIFF stack of n images28 Mb
Sequence3D-Double-HelixMT4.N2.HD-DH-Exp-as-listZIP, n TIFF images17 Mb
Sequence3D-Double-HelixMT4.N2.HD-DH-Exp-as-stackZIP, 1 TIFF stack of n images15 Mb
Sequence3D-Quad-plane (1 plane +150) (testing phase)MT4.N2.HD-QP+150-as-stackZIP, 1 TIFF stack of n images12 Mb
Sequence3D-Quad-plane (1 plane +450) (testing phase)MT4.N2.HD-QP+450-as-stackZIP, 1 TIFF stack of n images12 Mb
Sequence3D-Quad-plane (1 plane -150) (testing phase)MT4.N2.HD-QP-150-as-stackZIP, 1 TIFF stack of n images11 Mb
Sequence3D-Quad-plane (1 plane -450) (testing phase)MT4.N2.HD-QP-450-as-stackZIP, 1 TIFF stack of n images11 Mb

Parameters

Camera
Photon converter factor or Quantum efficiency (QE)0.90e-/Ph.
Resolution64pixels
Pixelsize100.00nm
Field of view6400.00nm
Optics
Wavelength660.00nm
Numerical aperture (NA)1.49
Autofluorescence
Poisson Distribution
Camera Noise
Read-out: Gaussian distribution74.4
EMGain: Gamma300.0
Spurious noise: Poisson distribution0.0020
Analog Digital Conversion
Total gain: QE*EM_gain/e_per_ADU6.00
Electron conversion e- per ADU45.00e-/DN
Baseline100.00DN
Saturation65535.00DN
Quantization16-bit
Computational Parameters
Thickness1500.00nm
Frames3020
MultithreadingOff-1 Thread
Pixelsize to PSF convolution10.0nm
Pixelsize for autofluorescence100.0nm
Pixelsize of the camera100.0nm
File formatTIFF 16-bits

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018