Real dataset • NPC-A647-3D
Real dataset Courtesy of Jonas Ries (EMBL)
TIFF Stacks are split across multiple files due to TIFF 4GB file size limit.
|Type||Modality||Link to Download||Format||Size|
|AS||NPC-A647-3D-BEADS-as-sequence.zip||Download as list of TIF images (ZIP)||7 Mb|
|AS||NPC-A647-3D-BEADS-as-stacks.tif||download as stack, split across 2 files||22 Mb|
|AS||NPC-A647-3D-stack_1.tif||download as stack, split across 2 files||3641 Mb|
|AS||NPC-A647-3D-stack_2.tif||download as stack, split across 2 files||3642 Mb|
Z axis: In order for easy inter-software comparison, please define frame 21 of 41 in the beads dataset as frame Z=0nm. Frame numbers smaller than the z = 0 frame number correspond to negative Z positions. This is an arbitrarily chosen zero for the z dimension of the coordinate system, and may not correspond to what you determine as the in-focus frame.
Drift: There is XY drift which requires correction for highest resolution. There is also 25 nm of Z drift which should be corrected.
Temporal grouping: Data should be temporally grouped for highest resolution.
Please indicate on submission:
Parameters of the sequence
Parameters of the z-stacks of beads
Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018