Real dataset • Tubulin-A647-3D
Real dataset Courtesy of Jonas Ries (EMBL)
Tubulin-A647-3D.zip may not be decompressable using the standard Windows unzip utility. In this case, use 7zip.
TIFF Stacks are split across multiple files due to TIFF 4GB file size limit.
|Type||Modality||Link to Download||Format||Size|
|AS||Tubulin-A647-3D-as-sequence.zip||Download as list of TIF images (ZIP)||14563 Mb|
|AS||Tubulin-A647-3D-BEADS-as-sequence.zip||Download as list of TIF images (ZIP)||82 Mb|
|AS||Tubulin-A647-3D-BEADS-as-stacks.tif||Download as stack, split across n files||210 Mb|
|AS||Tubulin-A647-3D-stacks_1.tif||Download as stack, split across n files||4377 Mb|
|AS||Tubulin-A647-3D-stacks_2.tif||Download as stack, split across n files||4377 Mb|
|AS||Tubulin-A647-3D-stacks_3.tif||Download as stack, split across n files||4377 Mb|
|AS||Tubulin-A647-3D-stacks_4.tif||Download as stack, split across n files||4377 Mb|
The first 4 frames in the Tubulin beads dataset are not in the correct Z-position (due to Z-piezo travelling time) and should be ignored. In any case they are also outside of the range of meaningful signal.
Z axis: In order for easy inter-software comparison, please define frame 201 of 401 in the beads dataset as frame Z=0nm. Frame numbers smaller than the z = 0 frame number correspond to negative Z positions. This is an arbitrarily chosen zero for the z dimension of the coordinate system, and may not correspond to what you determine as the in-focus frame.
Drift: There is XY drift which requires correction for highest resolution. There is also a small amount of Z drift (<5 nm) which can optionally be corrected.
Temporal grouping: Data should be temporally grouped for highest resolution.
Please indicate on submission:
Parameters of the sequence
Parameters of the z-stacks of beads
Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018