Single-Molecule Localization Microscopy  •  Software Benchmarking

Collection of reference datasets

The benchmarking of SMLM software package mainly relies on the usage of common reference datasets.

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Eye 2.0

Updated 01 February 2013

4 tubes of 1nm of radius in a field of view 38.4 * 38.4 μm.
Only 300 activated fluorophores in 40 frames.
Ideal dataset: no perturbation, no additional noise.

Download Ground-truth Send a email to get the login for the ground-truth

Acquisition Parameters

Camera
Photon converter factor or Quantum efficiency (QE)1.00e-/Ph.
QE = QE-Gain x QE-interacting [Ref. 1]
Resolution256pixels
Pixelsize150.00nm
Field of view38400.00nm
Optics
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Drift
DeriveDisable
ShakingDisable
Analog Digital Conversion
Electron conversion - Gain1.00DN/e-
Electron conversion - Offset0.00DN
Baseline100.00DN
Saturation16383.00DN
Quantization14-bit

Point-Spread Function (PSF)

Physical Model
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Offset focal plane 0nm
XY functionGaussian
FWHM258.21nm
Z functionExponential
Focal plane1 x FWHM at 0.0 nm
Defocus plane2 x FWHM at 500.0 nm

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018