Single-Molecule Localization Microscopy  •  Software Benchmarking

Collection of reference datasets

The benchmarking of SMLM software package mainly relies on the usage of common reference datasets.

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Snow 2.0

Updated 01 February 2013

Fluorophores are placed on a regular grid
Various depth and various number of photons
Field of view 50 μm x 50 μm

Download Ground-truth

Acquisition Parameters

Camera
Photon converter factor or Quantum efficiency (QE)1.00e-/Ph.
QE = QE-Gain x QE-interacting [Ref. 1]
Resolution450pixels
Pixelsize100.00nm
Field of view45000.00nm
Optics
Wavelength700.00nm
Numerical aperture (NA)1.40
Diffraction limit250.00nm
Drift
DeriveDisable
ShakingDisable
Analog Digital Conversion
Electron conversion - Gain1.00DN/e-
Electron conversion - Offset0.00DN
Baseline20.00DN
Saturation65535.00DN
Quantization16-bit

Point-Spread Function (PSF)

Physical Model
Wavelength700.00nm
Numerical aperture (NA)1.40
Diffraction limit250.00nm
Offset focal plane 0nm
ModelGibson and Lanni
Refractive index sample1.00
Refractive index immersion1.50
Offest working distance0.00nm

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018