Single-Molecule Localization Microscopy  •  Software Benchmarking

Collection of reference datasets

The benchmarking of SMLM software package mainly relies on the usage of common reference datasets.

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Bundled Tubes High Density

Updated 02 February 2013

Bundles of 8 tubes of 30 nm diameter
High-density, 81049 fluorophores on 168 frames

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Acquisition Parameters

Camera
Photon converter factor or Quantum efficiency (QE)1.00e-/Ph.
QE = QE-Gain x QE-interacting [Ref. 1]
Resolution64pixels
Pixelsize100.00nm
Field of view6400.00nm
Optics
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Drift
DeriveDisable
ShakingDisable
Analog Digital Conversion
Electron conversion - Gain1.00DN/e-
Electron conversion - Offset0.00DN
Baseline100.00DN
Saturation16383.00DN
Quantization14-bit

Point-Spread Function (PSF)

Physical Model
Wavelength723.00nm
Numerical aperture (NA)1.40
Diffraction limit258.21nm
Offset focal plane 0nm
XY functionGaussian
FWHM258.21nm
Z functionExponential
Focal plane1 x FWHM at 0.0 nm
Defocus plane2 x FWHM at 300.0 nm

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018