Single-Molecule Localization Microscopy  •  Software Benchmarking

Collection of reference datasets

The benchmarking of SMLM software package mainly relies on the usage of common reference datasets.


Tubulin • 2D • High Density

Courtesy of Nicolas Olivier and Debora Keller, LEB, EPFL

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Acquisition Parameters

Imaging conditions
Frame rate25fps
Image size64 x 64pixels
Number of frames500frames
Wavelength fluorescence690nm
CCD amplifier1 count~2 photonsno gain
Numerical aperture (NA)1.3(oil immersion objective)
Dark camera signal100

© 2018 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 30 Nov 2018