Collection of reference datasets
The benchmarking of SMLM software package mainly relies on the usage of common reference datasets, in particular synthetic datasets with known ground-truth.
Conditions of use These reference datasets are designed to be largely used by the developpers to validate software and by the users to check a software. Reference: Sage et al. Quantitative evaluation of software packages for SMLM, Nature Methods 12, 2015.
The dataset is a fixed cell, stained with mouse anti-alpha-tubulin primary antibody and Alexa647 secondary antibody.
The dataset consists in 9990 frames of 128x128 pixels stored as TIFF in 16 bits.
The intermittent increase in signal is due reactivation with a 405 nm laser.
Localization and rendering in 1280x1280 pxiels
Rendered image by the|
software Octane Rendered image by the
Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017