Benchmarking of SMLM Software
Comprehensive review of the single-molecule localization microscopy (SMLM, PALM, STORM) software packages.
This edition is focussed on three 3D localization modalities: astigmatism, biplane, or double-helix. The second edition of the challenge held in the Single Molecule Localization Microscopy Symposium (SMLMS) at Lausanne, Switzerland, August 28-30, 2016.Read more
In the SMLMS symposium, the special session of the Challenge will be live webcasted on a YouTube Channel, Sunday 28 August 2016, 15:00 CESTView Channel
News and views of Nature Photonics: Seamus Holden and Daniel Sage Nature Photonics 10, 2016.
This edition will be focussed on both 3D localization techniques. Broad participation is warmly encouraged!Read full PDF
D. Sage, H. Kirshner, T. Pengo, N. Stuurman, J. Min, S. Manley & M. Unser, Quantitative evaluation of software packages for single-molecule localization microscopy, Nature Methods 12, 717–724 (2015).Read full PDF
The Grand Challenge "Localization Microscopy" was presented as a workshop in the conference IEEE ISBI 2013 at San Francisco. Nearly 30 software have been run, mostly by the authors of the software, thus constituting the first world-wide effort in benchmarking the SMLM software. The results are published in this paper. The challenge is now re-open as a online permanent challenge.Read more
Super-resolution fluorescence microscopy is an emerging multidisciplinary field that has opened up new opportunities in studying the living cell at the nanometer scale. It overcomes the classical ...Read more
These challenges are the first attempt to foster the community to use the same common reference datasets to evaluate the performances of the localization software.
Collaboration through competition: Nature Methods Editorial, Nature Methods 11, 695 (2014).
Grand Challenge: These SMLM challenges are part of the Grand-Challenge in Biomedical Image Analysis.
This localization microscopy challenge goes hand in hand with the Open Bio Image Alliance (OBIA) mission. This consortium for bioimaging informatics aims at providing high-quality resources to bio ...Read more
Thanh-an Pham has a talk at the SMLMS 2017 Symposium in London: Developments of the ongoing 3D SMLM software challenge.
Update of the directory of SMLM software packages. 83 SMLM software are now identified and referred.
From April 10 to May 10 a second round of the challenge is running. All the DH have be submitted
with the new normalized datasets.
A large panel of software have submitted localization files to the challenge in all modalities.
The first dataset is released. The same sample is simulated in 4 modalities: 2D, 3D-astigmatism, 3D-biplane, and 3D-double-helix.
A new challenge will be held in August 2016. Send an email to be kept informed.
Advanced online publication of the comparative results on the Nature Methods website.
The ISBI Challenge is turned to a permanent online challenge.
Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Last update: 31 Mar 2017