The goal of this project is to extract the 3D structure of a biological sample that has been acquired by the bi-plane PALM (Photo-Activated localization Microscopy) method. At first, a theoretical model of the PSF (Point Spread Function) will be compared with experimental data of single beads. The experimental data will then be used for calibrating the two focal planes. The third stage would consist of deriving a 3D reconstruction algorithm that will be implemented in Matlab or in Java. The algorithm will be verified by both simulated and real data.
Collaboration: Prof Suliana Manley, LEB, EPFL