The Structured Illumination Microscopy (SIM) has been playing more and more important role in biomedical imaging. The SIM can break the diffraction limit of light, and achieve as twice resolution as the wide-filed fluorescence microscopy does. However, the SIM is very sensitive to the imperfection of the illumination pattern. Moreover, its commercial software (Zeiss, Nikon etc) is usually expensive to use. Based on our previous project, the main purposes of this project will be

1. To develop a fast and robust method for image calibration;
2. To overcome the drawbacks of the SIM, it is highly necessary to realize a new regularization method;
3. To our best knowledge, there is no public software containing the methods like

"SIM+calibration+regularization”. It will be of great interests for both researchers and microscope manufacturers to use our SIMageJ plugin.