Determination of the orientation of tiles obtained by parallel scanning

Marco Parisi

Semester Master Project

Microengineering section, EPFL

January 2010

Abstract

A new type of microscope performing parallel image data acquisition determines, by its construction, the parameters for the mosaicking of the acquired image data. The parameters for the reconstruction of the mosaic are thus instrument dependent and can, once determined, be reused to create mosaics with image data of subsequent acquisitions. This project presents an approach using image registration to determine the orientation of tiles in the mosaic reconstruction. For the registration, an existing least-squares point set fitting algorithm is adapted to the present problem. The results indicate that the hypotheses made on the mutual relative positions of the tiles in the mosaic have to be questioned.