Extended depth of field is an image-processing method to obtain in focus microscopic images of 3D objects and organisms. We freely provide a software to produce this in-focus image and the corresponding height map from a stack of images taken along the optical axis. Read the full information.
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To run this Applet, your browser should enable the Java Virtual Machine. Please wait a while collectiong applet and z-stack of images (5 Mb) |
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