Extended Depth of Field

Extended depth of field is an image-processing method to obtain in focus microscopic images of 3D objects and organisms. We freely provide a software to produce this in-focus image and the corresponding height map from a stack of images taken along the optical axis. Read the full information.

The images are courtesy of Nathalie Garin, ISREC, Lausanne.

The whole stack is composed of 20 color images of 1996x1450 pixels. Images shows mouse intestin's Peyer's patches.

The huge dataset of original images can be provided on request.