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BIG > Demos > Extended Depth of Field > Application case
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Extended Depth of Field

Extended Depth of Field

Extended depth of field is an image-processing method to obtain in focus microscopic images of 3D objects and organisms. We freely provide a software to produce this in-focus image and the corresponding height map from a stack of images taken along the optical axis. Read the full information.

Application case: Pancreas

The images are courtesy of Claude Bonnard, ISREC, Lausanne.

Stack of images of a pancreas.

The huge dataset of original images can be provided on request.

Original z-stack images presented as a QuickTime movie

Please download Apple's Quicktime.

 

Results of the Extended Depth of Field


Output

3-D view

Topology map