Extended Depth of Field

Extended depth of field is an image-processing method to obtain in focus microscopic images of 3D objects and organisms. We freely provide a software to produce this in-focus image and the corresponding height map from a stack of images taken along the optical axis. Read the full information.

Application case: Study of the retinal pigment epithelium

The images are courtesy of Peter Lundh von Leithner and Heba Ahmad, Institute of Ophthalmology, London.

The whole stack is composed of 4 color images of 1024x768 pixels.