Extended Depth of Field

Extended depth of field is an image-processing method to obtain in focus microscopic images of 3D objects and organisms. We freely provide a software to produce this in-focus image and the corresponding height map from a stack of images taken along the optical axis. Read the full information.

Mattheddleite

The images are courtesy of Rick Turner, British Micromount Society.

These images was obtained using an Olympus DP12 camera attached to a Wild M7A stereomicroscope. FoV is approx 1mm. The light blue is the mattheddleite, the dark blue linarite, and the white mostly leadhillite. The specimen comes from Red Gill Mine, in the English Lake District. The whole stack is composed of 10 color images of 1024x768 pixels.

z-stack images

Results of the Extended Depth of Field