3D Nanoscale Structures in Living Cells

Youri Marko

Semester Master Project

Microengineering section, EPFL

June 2010

Abstract

The goal of this semester project was to characterize the 3D structure of the endoplasmic reticulum. To achieve this target, the PSF (point spread function) of a PALM microscope has been modeled. By fitting this model on the datas, we extracted informations about the 3D localization of single fluorophores. The figure 1 shows the projection of the time sequence (5Õ000 images) obtained by a PALM microscope, the yellow window indicates the zone of interest that has been analyzed. The figure 2 shows single particles localizations inside the zone of interest.