Available Algorithms

The algorithms below are ready to be downloaded. They are generally written in JAVA or in ANSI-C, either by students or by the members of the Biomedical Imaging Group. Please contact the author of the algorithms if you have a specific question.

Check the conditions of use below.

JAVA: Plug-ins for ImageJ and Fiji

JAVA classes are usually meant to be integrated into the public-domain software ImageJ and into the image-processing package Fiji.

Circadian Gene Expression. This ImageJ plugin (CGE) is a semi-automatic tool to detect and track moving cell, and to measure the fluorescent protein expression level. CGE extracts the trajectory of the cells by tracking their displacements, makes the delineation of cell nucleus or whole cell, and finally yields measurements of various features, like reporter protein expression level, cell displacement.
DeconvolutionLab. DeconvolutionLab is a software package (ImageJ plugin) to deconvolve 2D or 3D microscopic images based on the knowledge of the PSF. It implements a variety of deconvolution algorithms: 1) Inverse filter, 2) Regularized inverse filter, 3) Landweber, 4) Threshold Landweber, 5) Tikhonov-Miller, 6) Richardson-Lucy, 7) Richardson-Lucy with TV Regularization. It also includes a convolution tool to generate simulated dataset with additive noise.
E-Snake. This ImageJ plugin (ESnake) implements a fast parametric active contour for segmenting nearly elliptic objects. ESnake outlines the targets using exponential B-splines, and it allows one to keep record of the curve with the ROI Manager from ImageJ.
Extended Depth of Focus. The extended depth of focus is a image-processing method to obtain in focus microscopic images of 3D objects and organisms. We freely provide a software as a plugin of ImageJ to produce this in-focus image and the corresponding height map of z-stack images.
Drop Shape Analysis. New method based on B-spline snakes (active contours) for measuring high-accuracy contact angles of sessile drops.
Fractional spline wavelet transform. This Java package computes the fractional spline wavelet transform of a signal or an image and its inverse.
Image Differentials. This Java class for ImageJ implements 6 operations based on the spatial differentiation of an image. It computes the pixel-wise gradient, Laplacian, and Hessian. The class exports public methods for horizontal and vertical gradient and Hessian operations (for those programmers who wish to use them in their own code).
Local Normalization. This Java plugin is able to segment an image using the watershed algorithm by directly flooding graylevel images. This implementation is in contrast with the classical approach working on the distance map image obtaining after thresholding. The grayscale watershed segmentation is useful to segment particles in contact when the model of shape is unknown a priori.
MIJ. MIJ offers the missing link between two imaging software: ImageJ/Fiji and Matlab. The goal of the package mij.jar is to provide static methods to exchange images and volumes. MIJ allows also to access to all built-in functions of ImageJ and to third-part plugins of ImageJ. MIJ is integrated in Fiji with a super-easy script to use it.
MonogenicJ. This ImageJ plugin performs multiresolution monogenic analyses of 2D images. It extracts wavelet-domain features that characterize the local orientation, the phase and the dominant frequency of an image patch at various levels of resolution.
MosaicJ. This Java class for ImageJ performs the assembly of a mosaic of overlapping individual images, or tiles. It provides a semi-automated solution where the initial rough positioning of the tiles must be performed by the user, and where the final delicate adjustments are performed by the plugin.
NeuronJ. This Java class for ImageJ was developed to facilitate the tracing and quantification of neurites in two-dimensional (2D) fluorescence microscopy images. The tracing is done interactively based on the specification of end points; the optimal path is determined on the fly from the optimization of a cost function using Dijkstra's shortest-path algorithm.
OrientationJ. The aim of this ImageJ plugin is to characterize the orientation and isotropy properties of a region of interest (ROI) in an image, based on the evaluation of the structure tensor in a local neighborhood. OrientationJ has four functionalities: visual representation of the orientation, quantitative orientation measurement, making distribution of orientations and corner detection (Harris Corner)
Ovuscule. The purpose of this ImageJ plugin is to detect elliptical bright blobs in images and to quantify them. It allows one to keep record of their location and size.
PixBleach. PixBleach is a ImageJ plugin which fits fluorescence photobleaching decay in the temporal sequence for every pixels. Three common decal models are available: the mono-exponential, the bi-exponential, and the stretched exponential. Bleach rate (time-constant) and other fitted parameters can be visualized as 32-bit image and exploited for further analysis.
PixFRET. The ImageJ plug-in PixFRET allows to visualize the FRET between two partners in a cell or in a cell population by computing pixel by pixel the images of a sample acquired in three channels.
Point Picker. This Java class for ImageJ allows the user to pick some points in an image and to save the list of pixel coordinates as a text file. It is also possible to read back the text file so as to restore the display of the coordinates.
PSF Generator. PSF Generator is a piece of software that allows to generate and visualize various 3D models of a microscope PSF. The current version has five different models: the Gaussian model, the simulated defocus, the scalar-based diffraction model Born & Wolf, the scalar-based diffraction model with 3 layers Gibson & Lanni, and finally, the vectorial-based model Richards & Wolf.
Resize. This ImageJ plugin changes the size of an image to any dimension using either interpolation, or least-squares approximation.
PureDenoise. The purpose of this ImageJ plugin is to propose a high-quality denoising algorithm of multidimensional fluorescence microscopy images (2D+t, 3D or color).
SheppLogan. The purpose of this ImageJ plugin is to generate sampled versions of the Shepp-Logan phantom. Their size can be tuned.
Snakuscule. The purpose of this ImageJ plugin is to detect circular bright blobs in images and to quantify them. It allows one to keep record of their location and size.
SpotDistance This ImageJ plugin determines intra-nuclear 3D cross-distances between fluorescent spots in multi-channel z-stack of image.
SpotTracker This ImageJ plugin is a robust and fast computational procedure for tracking fluorescent markers in time-lapse microscopy. The algorithm is optimized for finding the time-trajectory of single particles in very noisy image sequences.
StackReg. This Java class for ImageJ performs the recursive registration (alignment) of a stack of images, so that each slice acts as template for the next one.
SteerableJ. This ImageJ plugin implements a series of optimized contour and ridge detectors. The filters are steerable and are based on the optimization of a Canny-like criterion. They have a better orientation selectivity than the classical gradient or Hessian-based detectors.
TurboReg. This Java class for ImageJ performs the registration (alignment) of two images. The registration criterion is least-squares. The geometric deformation model can be translational, conformal, affine, and bilinear.
UnwarpJ. This Java class for ImageJ performs the elastic registration (alignment) of two images. The registration criterion includes a vector-spline regularization term to constrain the deformation to be physically realistic. The deformation model is made of cubic splines, which ensures smoothness and versatility.
Watershed. This Java plugin is able to segment an image using the watershed algorithm by directly flooding graylevel images. This implementation is in contrast with the classical approach working on the distance map image obtaining after thresholding. The grayscale watershed segmentation is useful to segment particles in contact when the model of shape is unknown a priori.

Matlab

Fractional spline wavelet transform. A Matlab package is available for computing the fractional spline wavelet transform of a signal or an image and its inverse.
Fractional spline and fractals. A Matlab package is available for computing the fractional smoothing spline estimator of a signal and for generating fBms (fractional Brownian motion). This spline estimator provides the minimum mean squares error reconstruction of a fBm (or 1/f-type signal) corrupted by additive noise.
Generalized Daubechies wavelets. This is a set of Matlab routines for computing generalized Daubechies wavelet filters.
MLTL deconvolution This Matlab package implements the MultiLevel Thresholded Landweber (MLTL) algorithm, an accelerated version of the TL algorithm that was specifically developped for deconvolution problems with a wavelet-domain regularization.
MRI Phantom This package is a collection of Matlab functions that implements analytical multi-channel MRI simulations of realistic phantoms.
OWT SURE-LET Denoising This Matlab package implements the interscale orthonormal wavelet thresholding algorithm based on the SURE-LET (Stein's Unbiased Risk Estimate/Linear Expansion of Thresholds) principle.
WSPM Wavelet-based statistical parametric mapping, a toolbox for SPM that incorporates powerful wavelet processing and spatial domain statistical testing for the analysis of fMRI data.

ANSI C

Most often, the ANSI-C pieces of code are not a complete program, but rather an element in a library of routines.

Affine transformation. This ANSI-C routine performs an affine transformation on an image or a volume. It proceeds by resampling a continuous spline model.
Registration. This ANSI-C routine performs the registration (alignment) of two images or two volumes. The criterion is least-squares. The geometric deformation model can be translational, rotational, and affine.
Shifted linear interpolation. This ANSI-C program illustrates how to perform shifted linear interpolation.
Spline interpolation. This ANSI-C program illustrates how to perform spline interpolation, including the computation of the so-called spline coefficients.
Spline pyramids. This software package implements the basic REDUCE and EXPAND operators for the reduction and enlargement of signals and images by factors of two based on polynomial spline representation of the signal.

Mathematica

E-splines. A Mathematica package is made available for the symbolic computation of exponential spline related quantities: B-splines, Gram sequence, Green function, and localization filter.

Maple

Hex-splines A novel spline family for hexagonal lattices. A Maple 7.0 worksheet is available for obtaining the analytical formula of any hex-spline (any order, regular, non-regular, derivatives, and so on).

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