Graphic STI
logo EPFL
text EPFL
english only
Biomedical Imaging Group
Student Projects
EPFL > BIG > Teaching > Student Projects > Completed Projects > David Nguyen
CONTENTS

Home page

News & Events

Seminars

People

Research

Publications

Tutorials & Reviews

Recent Talks

Demos

Download Algorithms

Jobs and Trainees

Teaching

Student Projects

Intranet

Transport-of-intensity approach for quantitative phase imaging

David Nguyen
Semester Project

Section of Microtechnics, EPFL

July 2013

 

Abstract

In this work, we consider the problem of imaging transparent (phase-only) objects by using a brightfield microscope. Our approach is built on the Transport of Intensity Equation (TIE) that relates the intensity of a scattered field to its phase distribution.

We introduce two different solutions of the TIE, namely Tikhonov and Total Variation (TV) regularization and propose two different implementation of the TV regularization, namely IRLS and FISTA. We confirm the ability of the TV regularization to retrieve sharper images with a better contrast. Also, we observe that the computation time of the FISTA is significantly shorter than IRLS.

We investigate the effect of the algorithm parameters with simulated data and we validate its applicability on real data.

Figure 1 HeLa cells at 40x magnification. a) brightfield image in focus and b) TIE phase reconstruction.

© 2022 EPFL • webmaster.big@epfl.ch • 11.08.2022