Graphic STI
logo EPFL
text EPFL
english only
Biomedical Imaging Group
Student Project: Cécile Bucher
BIG > Teaching > Student Project > Cécile Bucher

Home page

News & Events





Tutorials & Reviews

Recent Talks


Download Algorithms

Jobs and Trainees


Student Projects


Digital quantitative phase recovery from focal series of brightfield microscopy images

Cécile BucherSemester Project
Computer Science Section, EPFLJune 2008



The goal of this project is to recover digital quantitative phase from a focal series of brightŜeld microscopy images. When one want to observe biological material with a bright-field microscopy, staining can be used. Unfortunately, staining is not possible for living cells, or for materials that do not absorb dyes. In this case one can observe unstained specimens with a Differential Interference Contrast microscope (DIC) or a Phase Contrast microscope. The drawbacks of such tools are that they are very expensive and that they are only used for visualisation purposes.

The goal of this project, based on different papers, is therefore to implement a theoretical model that given a series of brightŜeld microscopy images will output the quantitative phase. Making this model work in practice will therefore lead to a very simple way to recover phase from unstainable specimens.

© 2022 EPFL • • 11.08.2022