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3D Deconvolution Microscopy
BIG >  3D Deconvolution Microscopy
DECONVOLUTION

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Synthetic hollow bars

These synthetic data consist of six parallel hollow bars. The images have been blurred using a theoretical microscopic PSF, and corrupted by Gaussian noise and Poisson noise with several signal to noise ratios (SNR = 15, 30 dB). The knowledge of the ground-truth allows one to quantatively compare the deconvolution tools.

Data

Statistics Mininum Maximum Mean μ Std deviation
Uncorrupted 0 65635 205.59 3664.85
G10-P15 0 65635 3527.12 4294.82
G10-P30 0 65635 5817.16 7514.97

PSF

PSF Features
Type Widefield
Refractive index ni: 1.518
Numerical aperture NA: 1.4
Spherical aberration W040: 0
Wavelength lamda: 500 nm
Spatial resolution deltar: 100 nm
Axial resolution deltaz: 200 nm
bars

Image size: 256x256 pixels

Number of z slices: 128 slices

Number of channels: 1

Dynamic: 16 bits

Image format: TIFF

Orthogonal preview

xy
XY section
zy
ZY section
xz
XZ section

Examples of deconvolution

example1 example1 example1
Input stack of images
Synthetic hollow bars
Corrupted data by blurring
and adding a Poisson noise
PSF
256x256x128 voxels
Output stack of images
Deconvolved dataset
Algorithm: Richardson-Lucy with TV regularization
40 iterations, lambda=0.001

© 2016 EPFL • webmaster.big@epfl.ch • 06.09.2016