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Biomedical Imaging Group
3D Deconvolution Microscopy
BIG >  3D Deconvolution Microscopy
DECONVOLUTION

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Open Software

» DeconvolutionLab2

» DeconvolutionLab

» PSF Generator

3D Reference Datasets

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Synthetic hollow bars

These synthetic data consist of six parallel hollow bars. The images have been blurred using a theoretical microscopic PSF, and corrupted by Gaussian noise and Poisson noise with several signal to noise ratios (SNR = 15, 30 dB). The knowledge of the ground-truth allows one to quantatively compare the deconvolution tools.

Dataset Series of 2D images Single z-stack (TIF)
Uncorrupted stack (reference) Bars.zip Bars-stack.zip
Blurred and additive (low) noisy stack Bars-G10-P15.zip Bars-G10-P15-stack.zip
Blurred and additive (high) noisy stack Bars-G10-P30.zip Bars-G10-P30-stack.zip
PSF PSF-Bars.zip PSF-Bars-stack.zip

Statistics

Statistics Mininum Maximum Mean μ Standard deviation
Size Image size: 256x256 pixels, 128 z-slices, 1 channel, TIFF, 16-bits
Uncorrupted 0 65635 205.59 3664.85
G10-P15 0 65635 3527.12 4294.82
G10-P30 0 65635 5817.16 7514.97

PSF

FeatureValue
Type Widefield
Refractive index ni: 1.518
Numerical aperture NA: 1.4
Spherical aberration W040: 0
Wavelength lamda: 500 nm
Spatial resolution deltar: 100 nm
Axial resolution deltaz: 200 nm

Views

xy xy
Maximum intensity projection of the reference 3 Orthogonal views of the reference
 
xy xy
Maximum intensity projection of the corrupted data 3 Orthogonal views of the corrupted data
 
xy xy
Maximum intensity projection of the corrupted data 3 Orthogonal views of the corrupted data
 
xy xy
Maximum intensity projection of the PSF 3 Orthogonal views of the PSF
 

© 2017 EPFL • webmaster.big@epfl.ch • 07.05.2017