One fluorescent bead

This test volume contains a fluorescent bead of known dimension—its diameter is precisely 2.5 μm. These data have the advantage of offering a simple object on which it is easy to perform a quantitative validation of the recovering of shape and dimension, before and after deconvolution.

Data

Download the stack: Bead.zip [14.7 Mb]

Statistics	Mininum	Maximum	Mean μ	Std deviation
Data	199	3743	339.27	174.29

PSF

Download the PSF: PSF-Bead.zip [1.9 Mb]

PSF Features
Type	Widefield
Refractive index	ni: 1.518
Numerical aperture	NA: 1.4
Spherical aberration	W040: 0
Wavelength	lamda: 530 nm
Spatial resolution	deltar: 64.5 nm
Axial resolution	deltaz: 160 nm

Image size: 256x256 pixels

Number of z slices: 256 slices

Number of channels: 1

Dynamic: 16 bits

Image format: TIFF

Orthogonal preview

XY section

ZY section

XZ section