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Biomedical Imaging Group
3D Deconvolution Microscopy
BIG >  3D Deconvolution Microscopy
DECONVOLUTION

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» DeconvolutionLab

» DeconvolutionLab2

» PSF Generator

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One fluorescent bead

This test volume contains a fluorescent bead of known dimension—its diameter is precisely 2.5 μm. These data have the advantage of offering a simple object on which it is easy to perform a quantitative validation of the recovering of shape and dimension, before and after deconvolution.

Data

  • Download the stack: Bead.zip [14.7 Mb]
Statistics Mininum Maximum Mean μ Std deviation
Data 199 3743 339.27 174.29

PSF

PSF Features
TypeWidefield
Refractive indexni: 1.518
Numerical apertureNA: 1.4
Spherical aberrationW040: 0
Wavelengthlamda: 530 nm
Spatial resolutiondeltar: 64.5 nm
Axial resolutiondeltaz: 160 nm
bead

Image size: 256x256 pixels

Number of z slices: 256 slices

Number of channels: 1

Dynamic: 16 bits

Image format: TIFF

Orthogonal preview

xy
XY section
zy
ZY section
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XZ section

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