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3D Deconvolution Microscopy
BIG >  3D Deconvolution Microscopy
DECONVOLUTION

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C. elegans embryo

This real dataset is composed of three stacks of images of a C. Elegans embryo. The deconvolution effects can be evaluated on different kinds of structures: extended objects (the chromosomes in the nuclei), filaments (the microtubules), and point-wise spots (a protein stained with CY3).

Data

Statistics Mininum Maximum Mean μ Std deviation
CY3 Channel 215 2842 992.34 595.23
FITC Channel 209 2929 657.66 327.59
DAPI Channel 205 2687 585.75 290.13

PSF

PSF Features
TypeWidefield
Refractive indexni: 1.518
Numerical apertureNA: 1.4
Spherical aberrationW040: 0
WavelengthDAPI:477nm; FITC:542nm; CY3:654nm
Spatial resolutiondeltar: 64.5 nm
Axial resolutiondeltaz: 160 nm
bio

Image size: 672x712 pixels

Number of z slices: 104 slices

Number of channels: 3

Dynamic: 16 bits

Image format: TIFF

Microscope Acquisition: Olympus CellR

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