C. elegans embryo

This real dataset is composed of three stacks of images of a C. Elegans embryo. The deconvolution effects can be evaluated on different kinds of structures: extended objects (the chromosomes in the nuclei), filaments (the microtubules), and point-wise spots (a protein stained with CY3).

Data

CY3 channel: CElegans-CY3.zip [65.2 Mb]
DAPI channel: CElegans-DAPI.zip [58.4 Mb]
FITC channel: CElegans-FITC.zip [59.9 Mb]

Statistics	Mininum	Maximum	Mean μ	Std deviation
CY3 Channel	215	2842	992.34	595.23
FITC Channel	209	2929	657.66	327.59
DAPI Channel	205	2687	585.75	290.13

PSF

CY3 channel: PSF-CElegans-CY3.zip [1.4 Mb]
DAPI channel: PSF-CElegans-DAPI.zip [1.0 Mb]
FITC channel: PSF-CElegans-FITC.zip [1.2 Mb]

PSF Features
Type	Widefield
Refractive index	ni: 1.518
Numerical aperture	NA: 1.4
Spherical aberration	W040: 0
Wavelength	DAPI:477nm; FITC:542nm; CY3:654nm
Spatial resolution	deltar: 64.5 nm
Axial resolution	deltaz: 160 nm

Image size: 672x712 pixels

Number of z slices: 104 slices

Number of channels: 3

Dynamic: 16 bits

Image format: TIFF

Microscope Acquisition: Olympus CellR

Objective: UPlanSApo 100X/1.4 oil(n.i. 1.518); image pixel size (binning 1X1): 64.5 nm
Laser intensity: 100% - Camera pixel size: 6450 nm - Gain: 0
z step: 200 nm (experiment 10, 1344x1024x111; crop 672x712x104)
Channel 1: DAPI, lamda excitation: 377/50 nm, lamda emission: 447/60 nm; Channel 2: FITC, lamda excitation: 485/20, lamda emission: 531/22; Channel 3: CY3,lamda excitation: 560/25,lamda emission: 634/40

Orthogonal preview

XY section

ZY section

XZ section